The northbound carriageway of the A9 near Pitlochry was closed following a collision involving a motorcycle and a van on Friday.
Traffic was brought to a standstill in both directions with witnesses reporting emergency services including fire crews, paramedics and an air ambulance in attendance.
Police confirmed a motorcyclist was injured in the crash and was taken to Ninewells Hospital in Dundee.
A Police Scotland spokesperson said: “Emergencies services attended at the A9 near to Pitlochry at a 3.15pm after a crash involving a motorcyclist and a van.
“The motorcyclist has been taken to Ninewells Hospital for treatment.”
The stretch was reopened at around 4.25pm.
Congestion was further impacted with a second two-vehicle incident close to the Inveralmond roundabout shortly before 4pm.
The road was fully reopened at around 6pm.