More than 200 Boots stores across the UK are under review.
The American owner of the high street chemist is reportedly looking at closing hundreds of stores across the UK.
A spokeswoman said the firm needed to be “agile” to adapt to the changing landscape.
More than 200 stores – around 10% of its UK standalone high street presence – are under review and could potentially be closed within two years.
Across the north and north-east, there are 30 branches – with 10 in Aberdeen alone, and three each in Elgin and Inverness.
Staff in Stonehaven, Peterhead, Fraserburgh, Nairn, Wick and Ullapool also add to the 60,000-strong workforce.
Boots has been part of Walgreen Boots Alliance (WBA) since 2012.
Profits for the health and beauty retailer fell by more than 18% last year prompting a drive to slash spending by £1.2 billion by April 2022.
While under-performing stores approaching the end of their lease could be closed, it’s understood WBA would mainly target areas with several stores.
The company, which also operates an opticians chain, would look to redeploy affected staff to another store in the area, sources said.
A Boots UK spokeswoman: “We currently do not have a major programme envisaged, but as you’d expect we always review under-performing stores and seek out opportunities for consolidation.
“As is natural with a business of our size, we have stores opening, closing and relocating on a regular basis, but we have had around 2,500 stores open for several years now.
“We are being realistic about the future and that we will need to be agile to adapt to the changing landscape.”
Last month WBA had said it was reviewing the Boots portfolio “focusing on low-performing stores and opportunities for consolidation”.
The news is the latest to come at a time when some of the biggest names on the high street have looked to restructure and cut costs.
Debenhams, Homebase and New Look have introduced measures to reduce rents across their UK footprint.
Just last week, Arcadia Group – which owns Top Shop, Dorothy Perkins and Burton among others – announced plans to close dozens of stores too.