Dion Donohue and Jacob Mellis have been suspended by Mansfield and will not be involved when the Stags host Leyton Orient.
The club announced on Monday that both midfielders had been punished following an alleged serious breach of club discipline. Neither player is allowed to attend training or be available for matches while Mansfield conducts an investigation.
Mellis was due to return to the squad after completing a three-match ban while Donohue has been building up his fitness.
Elsewhere, Stags boss John Dempster has a few players nursing knocks following a physical game at Carlisle at the weekend while defender Matt Preston is still suspended.
Orient will be without club captain Jobi McAnuff (groin), winger James Dayton (ankle) and defender Jamie Turley (hamstring).
The trio are yet to feature this season after injury problems in the summer or at the end of the last campaign.
Turley has resumed training but the trip to Nottinghamshire will come too soon, although it is hoped he will be back in action before the end of the month.
Orient are without a goal in their last three matches so interim head coach Ross Embleton may decide to make changes in attack.